Anti-ZNF266抗体(ab33005)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF266
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human ZNF266 aa 27-76 (N terminal). The exact sequence is proprietary.
    Sequence:

    EESRTVQRGDFQASEWKVQLKTKELALQQDVLGEPTSSGIQMIGSHNGGE


    Database link: Q14584

  • 阳性对照
    • HepG2 cell lysate, Human cerebellum.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1.5625e+006.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    May be involved in transcriptional regulation.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 14 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HZF1 antibody
    • KIAA2007 antibody
    • Zinc finger protein 1 antibody
    • Zinc finger protein 266 antibody
    • Zinc finger protein HZF1 antibody
    • ZN266_HUMAN antibody
    • ZNF 266 antibody
    • ZNF266 antibody
    see all

图片

  • Anti-ZNF266 antibody (ab33005) at 0.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size: 62 kDa
    Observed band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling ZNF266 with ab33005 at 4-8µg/ml. Arrows indicate positively labelled purkinje cells.

  • Anti-ZNF266 antibody (ab33005) at 1/1000 dilution + Lysates were prepared from human HepG2 cells at 25 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 62 kDa
    Observed band size: 25 kDa (why is the actual band size different from the predicted?)


    Exposure time: 2 minutes

    See Abreview

文献

ab33005 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HepG2 cells)
Loading amount
25 µg
Specification
HepG2 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Dr. Seth Frietze

Verified customer

提交于 Mar 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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