概述

  • 产品名称Anti-ZNF258抗体
  • 描述
    兔多克隆抗体to ZNF258
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Dog
  • 免疫原

    Synthetic peptide derived from residues 625 - 674 of human ZNF258.

    .

  • 阳性对照
    • WB: Jurkat cell lysate. IHC-P: Human kidney tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody is purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 79 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

Anti-ZNF258 antibody 图像

  • Anti-ZNF258 antibody (ab33004) at 0.5 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti Rabbit IgG 1/50000 - 1/100000

    Predicted band size : 79 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ZNF258 at 4-8µg/ml. Cells with positive label (indicated by arrows) are epithelial cells of renal tubule. Magnification: 400X.

Anti-ZNF258 antibody (ab33004)参考文献

ab33004 has not yet been referenced specifically in any publications.

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