Anti-ZNF239抗体(ab49431)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF239
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    14 amino acid synthetic peptide from within the a.a. region 41-90 of Human SNF239

  • 阳性对照
    • Jurkat cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab49431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312,500.

靶标

  • 功能
    May be involved in transcriptional regulation.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 9 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HOK 2 antibody
    • Kruppel zinc finger protein 2 antibody
    • MOK 2 antibody
    • MOK2 antibody
    • OTTHUMP00000019484 antibody
    • OTTMUSP00000024957 antibody
    • zfp 239 antibody
    • Zinc finger protein (C2H2) homologous to mouse MOK 2 antibody
    • Zinc finger protein 239 antibody
    • Zinc finger protein HOK 2 antibody
    • Zinc finger protein HOK-2 antibody
    • Zinc finger protein MOK 2 antibody
    • Zinc finger protein MOK-2 antibody
    • ZN239_HUMAN antibody
    • Znf239 antibody
    see all

图片

  • Anti-ZNF239 antibody (ab49431) at 0.5 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab49431 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (Multiple cell types)
Loading amount
30 µg
Specification
Multiple cell types
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Seth Frietze

Verified customer

提交于 Jul 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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