A region within synthetic peptide: SSDLTRFQDS MVNSFQFSKQ DDMPCQVDAG LSIIHVKTET SEGRTCKKSF, corresponding to N terminal amino acids 108-157 of Human ZNF225
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Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.3125 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Anti-ZNF225 antibody 图像
Western blot - ZNF225 antibody (ab66372)
Anti-ZNF225 antibody (ab66372) at 0.3125 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer
HRP conjugated anti-Rabbit IgGat 1/50,000 - 1/100,000 dilution
Predicted band size : 82 kDa
Observed band size : 82 kDa
Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.
Anti-ZNF225 antibody (ab66372)参考文献
has not yet been referenced specifically in any publications.
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