概述

  • 产品名称Anti-ZNF225抗体
    参阅全部 ZNF225 一抗
  • 描述
    兔多克隆抗体to ZNF225
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: SSDLTRFQDS MVNSFQFSKQ DDMPCQVDAG LSIIHVKTET SEGRTCKKSF, corresponding to N terminal amino acids 108-157 of Human ZNF225

  • 阳性对照
    • HepG2 cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab66372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3125 µg/ml. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能May be involved in transcriptional regulation.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 18 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C2 H2 type zinc finger protein antibody
    • Zinc finger protein 225 antibody
    • ZN225_HUMAN antibody
    • ZNF225 antibody

Anti-ZNF225 antibody 图像

  • Anti-ZNF225 antibody (ab66372) at 0.3125 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgGat 1/50,000 - 1/100,000 dilution

    Predicted band size : 82 kDa
    Observed band size : 82 kDa
    Additional bands at : 46 kDa. We are unsure as to the identity of these extra bands.

Anti-ZNF225 antibody (ab66372)参考文献

ab66372 has not yet been referenced specifically in any publications.

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