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RabMAb

Anti-ZNF217抗体[EPR6362] (ab124927)

概述

  • 产品名称
    Anti-ZNF217抗体[EPR6362]
    参阅全部 ZNF217 一抗
  • 描述
    兔单克隆抗体[EPR6362] to ZNF217
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues in the C-terminus of Human ZNF217.

  • 阳性对照
    • MCF7, PC3 and Caco2 cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab124927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 140 kDa (predicted molecular weight: 115 kDa).
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能
      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
    • 序列相似性
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ZABC 1 antibody
      • ZABC1 antibody
      • Zinc finger protein 217 antibody
      • ZN 217 antibody
      • ZN217 antibody
      • ZN217_HUMAN antibody
      • ZNF 217 antibody
      • ZNF217 antibody
      see all

    图片

    • All lanes : Anti-ZNF217 antibody [EPR6362] (ab124927) at 1/10000 dilution

      Lane 1 : MCF7 cell lysates
      Lane 2 : PC3 cell lysates
      Lane 3 : Caco2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit at 1/2000 dilution

      Predicted band size : 115 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    实验方案

    文献

    ab124927 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human ESCs)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    30 µg
    Specification
    human ESCs
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Jan 25 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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