本 快线抗体 仍未被充份测试和鉴定, 不在质保范围内, 具体请参见 使用条款及细节

概述

性能

应用

  • 应用说明

    This antibody gave a positive result in ELISA against the immunizing peptide (ab48177).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能
      Binds to the promoters of target genes and functions as repressor. Promotes cell proliferation and antagonizes cell death. Promotes phosphorylation of AKT1 at 'Ser-473'.
    • 序列相似性
      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 7 C2H2-type zinc fingers.
    • 细胞定位
      Nucleus.
    • Information by UniProt
    • 数据库链接
      • 别名
        • ZABC 1 antibody
        • ZABC1 antibody
        • Zinc finger protein 217 antibody
        • ZN 217 antibody
        • ZN217 antibody
        • ZN217_HUMAN antibody
        • ZNF 217 antibody
        • ZNF217 antibody
        see all

      图片

      This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

      • Western blot - Anti-ZNF217 antibody (ab48133)
        Western blot - Anti-ZNF217 antibody (ab48133)
        Anti-ZNF217 antibody (ab48133) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Nuclear Lysate at 8 µg

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Performed under reducing conditions.

        Predicted band size: 115 kDa
        Observed band size: 160 kDa (why is the actual band size different from the predicted?)

      文献

      This product has been referenced in:
      • Baran-Gale J  et al. An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells. RNA 22:1592-603 (2016). WB ; Human . Read more (PubMed: 27539783) »
      • Cohen PA  et al. The dark side of ZNF217, a key regulator of tumorigenesis with powerful biomarker value. Oncotarget 6:41566-81 (2015). Read more (PubMed: 26431164) »

      See all 3 Publications for this product

      发表研究结果有使用 ab48133？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

      提交评价 提交问题

      3 reviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-ZNF217 antibody

       Poor
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (human embryonic stem cell lines)
      Gel Running Conditions
      Reduced Denaturing (12%)
      Loading amount
      30 µg
      Specification
      human embryonic stem cell lines
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      提交于 Feb 02 2017

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ZNF217 antibody

       Poor
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Human Embryonic stem cell (HES2))
      Permeabilization
      Yes - 0.25% Trition X-100
      Specification
      Human Embryonic stem cell (HES2)
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
      Fixative
      Paraformaldehyde
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      提交于 Feb 02 2017

      Immunocytochemistry/ Immunofluorescence abreview for Anti-ZNF217 antibody

       Poor
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (human embryonic stem cells)
      Specification
      human embryonic stem cells
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
      Fixative
      Paraformaldehyde
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      提交于 Jan 26 2016

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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