概述

  • 产品名称Anti-ZNF207抗体
    参阅全部 ZNF207 一抗
  • 描述
    兔多克隆抗体to ZNF207
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 87-116 of Human ZNF207 (NP_001027464.1, NP_001091977.1).

  • 阳性对照
    • HepG2 cell line lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab123322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/10 - 1/50.
WB 1/100 - 1/500. Predicted molecular weight: 51 kDa.

靶标

Anti-ZNF207 antibody 图像

  • Immunofluorescence of A549 cells labelling ZNF207 with ab123322. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab123322 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF207 immunoreactivity is localized to the nucleus.

  • Anti-ZNF207 antibody (ab123322) at 1/100 dilution + HepG2 cell line lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 51 kDa

Anti-ZNF207 antibody (ab123322)参考文献

ab123322 has not yet been referenced specifically in any publications.

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