概述

  • 产品名称Anti-ZNF202抗体
    参阅全部 ZNF202 一抗
  • 描述
    兔多克隆抗体to ZNF202
  • 经测试应用适用于: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH conjugated synthetic peptide within central region amino acids 349 - 378 of Human ZNF202.

  • 阳性对照
    • WB: HepG2 cell line lysates IHC-P: Human breast carcinoma

性能

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应用

Our Abpromise guarantee covers the use of ab103034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000. Predicted molecular weight: 75 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/10 - 1/50.

靶标

  • 功能Transcriptional repressor that binds to elements found predominantly in genes that participate in lipid metabolism. Among its targets are structural components of lipoprotein particles (apolipoproteins AIV, CIII, and E), enzymes involved in lipid processing (lipoprotein lipase, lecithin cholesteryl ester transferase), transporters involved in lipid homeostasis (ABCA1, ABCG1), and several genes involved in processes related to energy metabolism and vascular disease.
  • 组织特异性Highly expressed in testis. Also expressed in breast carcinoma cell lines.
  • 序列相似性Contains 8 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
    Contains 1 SCAN box domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Zinc finger protein 202 antibody
    • Zinc finger protein with KRAB and SCAN domains 10 antibody
    • ZKSCAN 10 antibody
    • ZKSCAN10 antibody
    • ZN202_HUMAN antibody
    • ZNF 202 antibody
    • ZNF202 antibody
    see all

Anti-ZNF202 antibody 图像

  • Immunofluorescence of MDA-MB231 cells labelling ZNF202 with ab103034. MDA-MB231 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab103034 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZNF202 immunoreactivity is localized to the nucleus.

  • Immunoflurescence of HepG2 cells labelling ZNF202 with ab103034. An Alexa Fluor 488-conjugated Goat anti-rabbit IgG (green) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue).

  • Flow cytometry of HepG2 cells (right histogram) labelling ZNF202 with ab103034 compared to a negative control (left histogram). FITC-conjugated Goat anti-rabbit was used as the secondary antibody.

  • Anti-ZNF202 antibody (ab103034) at 1/100 dilution + HepG2 cell line lysates at 35 µg

    Predicted band size : 75 kDa
  • ab103034, at a 1/50 dilution, staining ZNF202 in formalin fixed and paraffin embedded Human breast carcinoma, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-ZNF202 antibody (ab103034)参考文献

ab103034 has not yet been referenced specifically in any publications.

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