概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF185
  • 特异性
    ab83100 recognizes ZNF185 variant 689.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide within residues corresponding to N terminal amino acids 144-193 (LAPYNIRRSS TSGDTEEEEE EEVVPFSSDE QKRRSEAASG VLRRTAPREH) of human ZNF185 (NP_009081).

  • 阳性对照
    • HeLa cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab83100 is purified by a peptide affinity chromatography method.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:12500.

靶标

  • 功能
    May be involved in the regulation of cellular proliferation and/or differentiation.
  • 组织特异性
    Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood.
  • 序列相似性
    Contains 1 LIM zinc-binding domain.
  • 细胞定位
    Cytoplasm > cytoskeleton. Cell junction > focal adhesion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LIM domain protein ZNF185 antibody
    • P1 A antibody
    • P1-A antibody
    • Zinc finger protein 185 (LIM domain) antibody
    • Zinc finger protein 185 antibody
    • ZN185_HUMAN antibody
    • ZNF185 antibody
    see all

Anti-ZNF185 antibody 图像

  • Anti-ZNF185 antibody (ab83100) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

实验方案

Anti-ZNF185 antibody (ab83100)参考文献

ab83100 has not yet been referenced specifically in any publications.

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