Anti-ZNF165抗体(ab86360)

概述

  • 产品名称Anti-ZNF165抗体
    参阅全部 ZNF165 一抗
  • 描述
    兔多克隆抗体to ZNF165
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 216-265 of Human ZNF165;

    ISEASGESQDICKSAGRVKRQWEKESGESQRLSSAQDEGFGKILTHKNTV

    ; NP_003438

  • 阳性对照
    • HeLa cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab86360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能May be involved in transcriptional regulation.
  • 组织特异性Expressed specifically in testis.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
    Contains 1 SCAN box domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 53 antibody
    • CT53 antibody
    • LD65 antibody
    • MGC140342 antibody
    • OTTHUMP00000016195 antibody
    • Zinc finger and SCAN domain containing protein 7 antibody
    • Zinc finger and SCAN domain-containing protein 7 antibody
    • Zinc finger protein 165 antibody
    • ZINC FINGER PROTEIN 165; ZNF165 antibody
    • ZN165_HUMAN antibody
    • ZNF165 antibody
    • ZPF165 antibody
    • ZSCAN7 antibody
    see all

Anti-ZNF165 antibody 图像

  • Anti-ZNF165 antibody (ab86360) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling ZNF165 with ab86360 at 5.0µg/ml.

Anti-ZNF165 antibody (ab86360)参考文献

ab86360 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (breast cancer cell line)
Specification breast cancer cell line
Treatment siZNF165 for 72hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Ms. Jennifer Macion

Verified customer

提交于 Mar 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"