概述

  • 产品名称
  • 描述
    兔多克隆抗体to ZNF161
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region from internal sequence amino acids 288-337 (

    SCNICGKLLSAAYITSHLKTHGQSQSINCNTCKQGISKTCMSEETSNQKQ

    ) of Human ZNF161 (NP_009077).

  • 阳性对照
    • 293T Cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab85414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.
  • 组织特异性
    Ubiquitously expressed. Highest levels in skeletal muscle and kidney.
  • 序列相似性
    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DB1 antibody
    • MGC82370 antibody
    • Putative transcription factor DB1 antibody
    • Vascular endothelial zinc finger 1 antibody
    • VEZF1 antibody
    • VEZF1_HUMAN antibody
    • Zinc finger protein 161 antibody
    • ZNF161 antibody
    see all

图片

  • Anti-ZNF161 antibody (ab85414) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 57 kDa
    Observed band size : 57 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling ZNF161 with ab85414 at 5µg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling ZNF161 with ab85414.



  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Adult Placenta Lysate


  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Fetal Liver Lysate


  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Fetal Lung Lysate


  • Predicted band size : 57 kDa
    Western Blot using ab85414 at 1.0ug/ml. Sample type: Hum. Fetal Muscle Lysate

文献

This product has been referenced in:
  • Gerald D  et al. RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription. Nat Commun 4:2824 (2013). Read more (PubMed: 24280686) »

See 1 Publication for this product

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