Anti-ZNF143抗体(ab58168)

概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to ZNF143
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-627 of Human ZNF143

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

性能

应用

Our Abpromise guarantee covers the use of ab58168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 68 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 相关性
    ZNF143 is a human homolog of Xenopus transcriptional activator staf that is involved in selenocystyl tRNA transcription which binds to the SPH motif of small nuclear RNA (snRNA) gene promoters.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • MGC66276 antibody
    • mStaf antibody
    • pHZ 1 antibody
    • SBF antibody
    • Selenocysteine tRNA gene transcription activating factor antibody
    • SPH binding factor antibody
    • STAF antibody
    • Zfp143 antibody
    • zgc:66276 antibody
    • Zinc finger protein 143 antibody
    see all

图片

  • ZNF143 antibody (ab58168) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.


  • Predicted band size : 68 kDa
    ZNF143 antibody (ab58168) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab58168 at 10 ug/ml staining ZNF143 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

文献

ab58168 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing (4-10% gel)
Loading amount
30 µg
Treatment
siRNA
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Alina Radziwon

Verified customer

提交于 Nov 09 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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