概述

  • 产品名称Anti-ZNF133抗体
    参阅全部 ZNF133 一抗
  • 描述
    小鼠单克隆抗体to ZNF133
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 121-231 of human ZNF133 (NP_003425) with a 26 kDa tag.

  • 阳性对照
    • Hela and SJCRH30 cell lysates

性能

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应用

Our Abpromise guarantee covers the use of ab77461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 73 kDa.
ELISA Use at an assay dependent dilution. Detection limit for this product is ~30 ng/ml when used as a capture antibody.

靶标

  • 功能May be involved in transcriptional regulation as a repressor.
  • 组织特异性Seems ubiquitous. Seen in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 15 C2H2-type zinc fingers.
    Contains 1 KRAB domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • pHZ 13 antibody
    • pHZ 66 antibody
    • Zinc finger protein 133 (clone pHZ13) antibody
    • Zinc finger protein 133 antibody
    • Zinc finger protein 150 (pHZ66) antibody
    • Zinc finger protein 150 antibody
    • ZN133_HUMAN antibody
    • ZNF133 antibody
    • ZNF150 antibody
    see all

Anti-ZNF133 antibody 图像

  • Anti-ZNF133 antibody (ab77461) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 73 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-ZNF133 antibody (ab77461) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • Anti-ZNF133 antibody (ab77461) at 1/500 dilution + SJCRH30 cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 73 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

Anti-ZNF133 antibody (ab77461)参考文献

ab77461 has not yet been referenced specifically in any publications.

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