Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide: K PEVVDSTE SI PVSDEDS DAM VDDPND EDFV PFRPR RSPRM SLRS SVSQRA, corresponding to amino acids 250-300 of Human ZMYM3
Extract from HeLa cell extracts.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 8mM Tris, 60mM Citrate, pH 7-8
Concentration information loading...
Immunogen affinity purified
This antibody was purified using an epitope specific to ZNF261 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 152 kDa).
1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Most abundant in brain, moderate in muscle and heart, low in other tissues except placenta.
Note=A chromosomal aberration involving ZMYM3 may be a cause of X-linked mental retardation in Xq13.1. Translocation t(X;13)(q13.1;?).
Contains 9 MYM-type zinc fingers.
Information by UniProt
There are 2 isoforms produced by alternative splicing.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYM3 antibody (ab19165)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma tissue labelling ZMYM3 with ab19165 at 1/5000 (0.2µg/ml). Detection: DAB.
Western blot - Anti-ZMYM3 antibody (ab19165)
Ab19165 at 0.1 ug/ml staining ZNF261 in 10 ug of Nuclear extract from HeLa cells by Western blot. Detection was by chemiluminescence with an exposure time of 15 minutes. Ab19165 at 0.1 ug/ml staining ZMYM3 in 10 ug of Nuclear extract from HeLa cells by Western blot. Detection was by chemiluminescence with an exposure time of 15 minutes.
has not yet been referenced specifically in any publications.
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