ZM549865 Fluorescent ligand (Red) (ab118650)

概述

  • 产品名称ZM549865 Fluorescent ligand (Red)
  • 描述

    Fluorescent 5-HT1B antagonist

  • 生物学描述Fluorescent 5-HT1B antagonist (pKD = 6.71). No intrinsic agonist activity in vitro. Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics.

    Excitation wavelength: 633 nm

    Emission wavelength: 650 nm

  • 纯度> 97%

性能

    应用

    Our Abpromise guarantee covers the use of ab118650 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Small Molecule Fluorescent Optical Detection Use at an assay dependent concentration.
    Receptor Localization Use at an assay dependent concentration.
    Fluorescent Cell Imaging Use at an assay dependent concentration.

    ZM549865 Fluorescent ligand (Red) 图像

    • ab118650 ligand binding and displacement. Left: The ab118650 ligand (100 nM) binding to CHO cells expressing 5-HT1B receptors. Center: Binding in the same 5-HT1B expressing cell line blocked by the unlabelled competitor CP-94253 at 1 µM. Right: Lack of strong binding of ab118650 to CHO cells expressing 5-HT1A receptors. Nuclei have been counter-stained with Hoechst.
    • To determine the apparent KD for ab118650, cells were treated with varying concentrations of 5-CT or CP-94253 alone, or in the presence of 1 µM ab118650, and the cyclic-AMP induced expression of SPAP measured. The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118650, compared to the response curve for the agonist alone. Similar studies were also carried out using a CHO cell line expressing the Human 5-HT1A receptor to test for receptor selectivity. ab118650 did not show either agonist or antagonist activity at the Human 5-HT1A receptor. Antagonist –log KD values for 5-HT1B 6.71 (vs 5-CT)and 6.86 (vs CP-94253)
    • To determine the apparent KD for ab118650, cells were treated with varying concentrations of 5-CT or CP-94253 alone, or in the presence of 1 uM ab118650, and the cyclic-AMP induced expression of SPAP measured. The apparent KD was calculated from the rightward shift of the agonist response curve in the presence of ab118650, compared to the response curve for the agonist alone. Similar studies were also carried out using a CHO cell line expressing the Human 5-HT1A receptor to test for receptor selectivity. ab118650 did not show either agonist or antagonist activity at the Human 5-HT1A receptor. Antagonist –log KD values for 5-HT1A 4.09 (vs 5-CT) and <6.00(vs CP-94253)

    ZM549865 Fluorescent ligand (Red) (ab118650)参考文献

    ab118650 has not yet been referenced specifically in any publications.

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