Recombinant fragment, corresponding to a region within amino acids 1-364 of Human ZNF306 (NP_077819).
WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cells
ICC/IF: HeLa cell
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 61 kDa.
1/100 - 1/200.
Acts as a transcriptional regulator. Binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3'. Associates with chromatin at the ITGB4 and VEGF promoters. Activates the transcription of genes associated with colon cancer progression.
Expressed in colorectal cancers (at protein level).
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers. Contains 1 KRAB domain. Contains 1 SCAN box domain.
Information by UniProt
SCAN-KRAB-zinc finger protein antibody
Western blot - Anti-ZKSCAN3 antibody (ab96731)
All lanes : Anti-ZKSCAN3 antibody (ab96731) at 1/1000 dilution Lane 1 : HeLa S3 whole cell lysate Lane 2 : HepG2 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 61 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-ZKSCAN3 antibody (ab96731)
Immunofluorescence analysis of ZNF306 in paraformaldehyde fixed HeLa, using ab96731 at a 1/200 dilution.
Lower image (2): merged with DNA probe.
has not yet been referenced specifically in any publications.
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