The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
应用说明Is unsuitable for ICC/IF or IP.
功能Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. Contains 1 protein kinase domain.
翻译后修饰Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus. Autophosphorylated. Phosphorylated by ROCK1.
细胞定位Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
Western blot - ZIP Kinase antibody [EPR1636Y] (ab51602)
Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 53 kDa Observed band size : 53 kDa
Anti-ZIP Kinase antibody [EPR1636Y] (ab51602)参考文献
This product has been referenced in:
Marina O et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res70:1344-55 (2010).
Read more (PubMed: 20124481) »