使用敲除细胞株进行验证RabMAb

Anti-ZIP Kinase抗体[EPR1636Y] (ab51602)

概述

  • 产品名称Anti-ZIP Kinase抗体[EPR1636Y]
    参阅全部 ZIP Kinase 一抗
  • 描述
    兔单克隆抗体[EPR1636Y] to ZIP Kinase
  • 特异性ab51602 recognizes ZIP Kinase.
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC/IF or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human ZIP Kinase aa 400 to the C-terminus (C terminal).
    Database link: O43293

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC/IF or IP.
  • 靶标

    • 功能Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
      Autophosphorylated. Phosphorylated by ROCK1.
    • 细胞定位Nucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DAP kinase 3 antibody
      • DAP like kinase antibody
      • DAP-like kinase antibody
      • Dapk 3 antibody
      • DAPK3 antibody
      • DAPK3_HUMAN antibody
      • Death associated kinase 3 antibody
      • Death associated protein kinase 3 antibody
      • Death-associated protein kinase 3 antibody
      • Dlk antibody
      • EC 2.7.11.1 antibody
      • FLJ36473 antibody
      • MYPT1 kinase antibody
      • ZIP antibody
      • ZIP kinase antibody
      • ZIP kinase isoform antibody
      • ZIP-kinase antibody
      • ZIPK antibody
      • zipper-interacting protein kinase antibody
      see all

    Anti-ZIP Kinase antibody [EPR1636Y] 图像



    • Predicted band size : 53 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: ZIP Kinase knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: A431 whole cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51602 observed at 53 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab51602 was shown to specifically react with ZIP Kinase when ZIP Kinase knockout samples were used. Wild-type and ZIP Kinase knockout samples were subjected to SDS-PAGE. Ab51602 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-ZIP Kinase antibody [EPR1636Y] (ab51602) at 1/1000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 53 kDa
      Observed band size : 53 kDa

    Anti-ZIP Kinase antibody [EPR1636Y] (ab51602)参考文献

    This product has been referenced in:
    • Kocher BA  et al. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res 13:358-67 (2015). WB ; Human . Read more (PubMed: 25304685) »
    • Marina O  et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res 70:1344-55 (2010). WB ; Human . Read more (PubMed: 20124481) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (Heart)
    Gel Running Conditions Reduced Denaturing (12% acrylamide)
    Loading amount 25 µg
    Specification Heart
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 May 11 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"