兔多克隆抗体to Zinc finger MIZ domain-containing蛋白1
ab113294 antibody will recognize all three known Zinc finger MIZ domain-containing protein 1 isoforms. It is predicted to not cross-react with other PIAS protein family members or other Human proteins based on sequence homology.
Synthetic peptide corresponding to 15 amino acids near the N terminal of Human Zinc finger MIZ domain-containing protein 1
A562 cell lysate tested by WB.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa.
Use a concentration of 10 µg/ml.
Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation.
Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.
Contains 1 SP-RING-type zinc finger.
The C-terminal proline-rich domain possesses a significant intrinsic transcriptional activity. This activity is inhibited by the N-terminus in the full-length protein.
Nucleus speckle. Cytoplasm.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab113294)
Immunofluorescence of Zinc finger MIZ domain-containing protein 1 in K562 cells with ab113294 at 20 ug/mL.
Western blot - Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab113294)
All lanes : Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab113294) at 0.5 µg/ml Lane 1 : K562 cell lysate without blocking peptide Lane 2 : K562 cell lysate with blocking peptide Lysates/proteins at 15 µg per lane. Predicted band size: 115 kDa
Immunocytochemistry - Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab113294)
Immunocytochemical analysis of K562 cells, staining Zinc finger MIZ domain-containing protein 1 with ab113294 at 10 µg/ml.
has not yet been referenced specifically in any publications.
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