Anti-Zinc finger MIZ domain-containing蛋白1抗体(ab113294)
Key features and details
- Rabbit polyclonal to Zinc finger MIZ domain-containing protein 1
- Suitable for: ICC/IF, WB, ICC
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Zinc finger MIZ domain-containing蛋白1抗体
参阅全部 Zinc finger MIZ domain-containing protein 1 一抗 -
描述
兔多克隆抗体to Zinc finger MIZ domain-containing蛋白1 -
宿主
Rabbit -
特异性
ab113294 antibody will recognize all three known Zinc finger MIZ domain-containing protein 1 isoforms. It is predicted to not cross-react with other PIAS protein family members or other Human proteins based on sequence homology. -
经测试应用
适用于: ICC/IF, WB, ICCmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to 15 amino acids near the N terminal of Human Zinc finger MIZ domain-containing protein 1
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阳性对照
- A562 cell lysate tested by WB.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab113294于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa.
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ICC |
Use a concentration of 10 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 115 kDa. |
ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Increases ligand-dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation. -
组织特异性
Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes. -
序列相似性
Contains 1 SP-RING-type zinc finger. -
结构域
The C-terminal proline-rich domain possesses a significant intrinsic transcriptional activity. This activity is inhibited by the N-terminus in the full-length protein. -
细胞定位
Nucleus speckle. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 57178 Human
- Omim: 607159 Human
- SwissProt: Q9ULJ6 Human
- Unigene: 193118 Human
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别名
- hZIMP10 antibody
- KIAA1224 antibody
- MIZ antibody
see all
图片
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Immunofluorescence of Zinc finger MIZ domain-containing protein 1 in K562 cells with ab113294 at 20 ug/mL.
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All lanes : Anti-Zinc finger MIZ domain-containing protein 1 antibody (ab113294) at 0.5 µg/ml
Lane 1 : K562 cell lysate without blocking peptide
Lane 2 : K562 cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 115 kDa -
Immunocytochemical analysis of K562 cells, staining Zinc finger MIZ domain-containing protein 1 with ab113294 at 10 µg/ml.
实验方案
数据表及文件
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Datasheet download
文献 (0)
ab113294 尚未被引用在任何文献中。