概述

  • 产品名称Anti-Zinc Alpha 2 Glycoprotein抗体
    参阅全部 Zinc Alpha 2 Glycoprotein 一抗
  • 描述
    兔多克隆抗体to Zinc Alpha 2 Glycoprotein
  • 特异性This antibody is specific for Zinc Alpha 2 Glycoprotein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human Zinc Alpha 2 Glycoprotein. 278 amino acid residues of the human ZA2G and 12 additional amino acid residues. Source of immunogen, 293 cell line (Human embryonic kidney)

  • 阳性对照
    • Human serum

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 0.1M Sodium chloride, 0.05M PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Immunoaffinity chromatography on a column with immobilized recombinant human Zinc Alpha 2 Glycoprotein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.

靶标

  • 功能Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
  • 组织特异性Blood plasma, seminal plasma, urine, saliva, sweat, epithelial cells of various human glands, liver.
  • 序列相似性Belongs to the MHC class I family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha 2 glycoprotein 1 zinc antibody
    • Alpha 2 glycoprotein 1 zinc binding antibody
    • Alpha 2 glycoprotein zinc antibody
    • Azgp1 antibody
    • ZA2G antibody
    • ZA2G_HUMAN antibody
    • ZAG antibody
    • Zinc alpha 2 glycoprotein precursor antibody
    • Zinc-alpha-2-glycoprotein antibody
    • Zn alpha 2 glycoprotein antibody
    • Zn alpha 2 GP antibody
    • Zn alpha2 glycoprotein antibody
    • Zn-alpha-2-glycoprotein antibody
    • Zn-alpha-2-GP antibody
    • ZNGP1 antibody
    see all

Anti-Zinc Alpha 2 Glycoprotein antibody 图像

  • ab38929 (4µg/ml) staining MMP1 - Hemopexin domain in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of epithelial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-Zinc Alpha 2 Glycoprotein antibody (ab47116) at 1 µg/ml

    Lane 1 : Human Plasma Total Protein Lysate
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

Anti-Zinc Alpha 2 Glycoprotein antibody (ab47116)参考文献

This product has been referenced in:
  • Shetty V  et al. Investigation of ovarian cancer associated sialylation changes in N-linked glycopeptides by quantitative proteomics. Clin Proteomics 9:10 (2012). WB . Read more (PubMed: 22856521) »
  • Liu Z  et al. Tandem 18O stable isotope labeling for quantification of N-glycoproteome. J Proteome Res 9:227-36 (2010). WB ; Human . Read more (PubMed: 19921957) »

See all 2 Publications for this product

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