This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

概述

  • 产品名称Anti-Zic2抗体
    参阅全部 Zic2 一抗
  • 描述
    山羊多克隆抗体to Zic2

    This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »

  • 特异性ab121036 is not expected to cross-react with other Zic proteins.
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-HSGLSSNFNEWY

    by a Cysteine residue linker, corresponding to C terminal amino acids 520-531 of Human Zic2 (NP_009060.2).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab121036 is purified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kDa according to NP_009060.2 and 58.6kDa for the ZIC2-Myc protein. We have observed an approx 75kDa band in Human Brain (Cerebellum and Cerebral Cortex) lysates .

靶标

  • 功能Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
  • 疾病相关Defects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5) [MIM:609637]. A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces.
  • 序列相似性Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 结构域The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • 翻译后修饰Phosphorylated.
    Ubiquitinated by RNF180, leading to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HPE 5 antibody
    • HPE5 antibody
    • Odd paired homolog Drosophila antibody
    • Zic 2 antibody
    • Zic family member 2 (odd paired Drosophila homolog) antibody
    • Zic family member 2 antibody
    • ZIC2 antibody
    • ZIC2_HUMAN antibody
    • Zinc finger protein of the cerebellum 2 antibody
    • Zinc finger protein ZIC 2 antibody
    • Zinc finger protein Zic2 antibody
    see all

Anti-Zic2 antibody 图像

This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

  • RWPE1 lysate (60ug protein in RIPA buffer) overexpressing Human ZIC2 with C-terminal MYC tag probed with ab121036 (0.5ug/ml) in the right panel(B) and probed with anti-MYC Tag (1/1000) and anti-beta-Actin in the left panel (A). Mock-transfected RWPE1 in lanes 1 and expressing GFP in lanes 2. Primary incubations were overnight at 4C. Detected by chemiluminescence.

Anti-Zic2 antibody (ab121036)参考文献

ab121036 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"