Anti-Zic2抗体(ab10550)

概述

  • 产品名称Anti-Zic2抗体
    参阅全部 Zic2 一抗
  • 描述
    兔多克隆抗体to Zic2
  • 特异性Recognises a band at about 60kD in mouse brain lysate (but not in rat brain lysate). A lower band is also detected, the identity of this is unknown.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Cow, Human, Zebrafish
  • 免疫原

    Synthetic peptide:

    GRSEQYRQVASPRTDPYS

    conjugated to KLH, corresponding to amino acids 190-207 of Mouse Zic2.

性能

应用

Our Abpromise guarantee covers the use of ab10550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).

靶标

  • 功能Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos.
  • 疾病相关Defects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5) [MIM:609637]. A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces.
  • 序列相似性Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 结构域The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • 翻译后修饰Phosphorylated.
    Ubiquitinated by RNF180, leading to its degradation.
  • 细胞定位Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression. Both phosphorylated and unphosphorylated forms are localized in the nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HPE 5 antibody
    • HPE5 antibody
    • Odd paired homolog Drosophila antibody
    • Zic 2 antibody
    • Zic family member 2 (odd paired Drosophila homolog) antibody
    • Zic family member 2 antibody
    • ZIC2 antibody
    • ZIC2_HUMAN antibody
    • Zinc finger protein of the cerebellum 2 antibody
    • Zinc finger protein ZIC 2 antibody
    • Zinc finger protein Zic2 antibody
    see all

Anti-Zic2 antibody 图像



  • Predicted band size : 55 kDa


    Western blot using ab10550 at 1/500.

    Lane 1 : Mouse Brain tissue lysate
    Lane 2 : Rat Brain tissue lysate

    The upper band in the mouse brain lysate represents Zic2.

    Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
    Exposure time: 5 minutes
    Expected molecular weight: 55 kDa
    20µg of cell lysate per lane

Anti-Zic2 antibody (ab10550)参考文献

ab10550 has not yet been referenced specifically in any publications.

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As you have noticed, the Anti-Zic2 antibody (ab10550) has as yet not been tested in IP. This does not necessarily mean that it would not work, simply that we have not tested it and would not be able to guarantee i...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse forebrain E17)
Specification Mouse forebrain E17
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - 0.025% TX100
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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提交于 Aug 21 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"