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Our Abpromise guarantee covers the use of ab10550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).|
Western blot using ab10550 at 1/500.
Lane 1 : Mouse Brain tissue lysate
Lane 2 : Rat Brain tissue lysate
The upper band in the mouse brain lysate represents Zic2.
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 5 minutes
Expected molecular weight: 55 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"