概述

  • 产品名称Anti-Zic1抗体
    参阅全部 Zic1 一抗
  • 描述
    兔多克隆抗体to Zic1
  • 特异性ab72694 detects endogenous levels of total Zic1 protein.
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human Zic1

  • 阳性对照
    • Jurkat and COLO cell extracts. Paraffin embedded human brain tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab72694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20713527
WB 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 23603813

靶标

  • 功能Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally-located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'.
  • 组织特异性CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.
  • 序列相似性Belongs to the GLI C2H2-type zinc-finger protein family.
    Contains 5 C2H2-type zinc fingers.
  • 结构域The C2H2-type 3, 4 and 5 zinc finger domains are necessary for transcription activation.
  • 细胞定位Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Odd paired homolog Drosophila antibody
    • Zic 1 antibody
    • ZIC antibody
    • Zic family member 1 (odd-paired Drosophila homolog) antibody
    • Zic family member 1 antibody
    • Zic protein member 1 antibody
    • zic1 antibody
    • ZIC1_HUMAN antibody
    • Zinc finger protein 201 antibody
    • Zinc finger protein of the cerebellum 1 antibody
    • Zinc finger protein ZIC 1 antibody
    • Zinc finger protein ZIC1 antibody
    • ZNF 201 antibody
    • ZNF201 antibody
    see all

Anti-Zic1 antibody 图像

  • All lanes : Anti-Zic1 antibody (ab72694) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COLO cell extract
    Lane 3 : Jurkat cell extract with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • ab72694 at 1/50 dilution staining Zic1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Anti-Zic1 antibody (ab72694)参考文献

This product has been referenced in:

See all 3 Publications for this product

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