Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 906-956 of Human ZHX3 (NP_055850.1)
HeLa and 293T whole cell lysates
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 105 kDa.
Use a concentration of 2 - 5 µg/ml.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Acts as a transcriptional repressor. Is a regulator of podocyte gene expression during primary glomerula disease.
Widely expressed. High expression in kidney.
Belongs to the ZHX family.
Contains 2 C2H2-type zinc fingers. Contains 5 homeobox DNA-binding domains.
Information by UniProt
Triple homeobox 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZHX3 antibody (ab99353)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling ZHX3 with ab99353 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - Anti-ZHX3 antibody (ab99353)
All lanes : Anti-ZHX3 antibody (ab99353) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 105 kDa Exposure time: 30 seconds
Immunoprecipitation - Anti-ZHX3 antibody (ab99353)
Detection of Human ZHX3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). ab99353 used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. ZHX3 was also immunoprecipitated by another rabbit anti-ZHX3 antibody (lane 2) and control IgG (lane 3). Detection Chemiluminescence with exposure times of 3 seconds.
has not yet been referenced specifically in any publications.
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