概述

  • 产品名称
    Anti-Zhangfei抗体
    参阅全部 Zhangfei 一抗
  • 描述
    兔多克隆抗体to Zhangfei
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Zhangfei.

    (Peptide available as ab28713.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: 293T Embryonic Kidney cell line (Human), HepG2 (Human hepatocellular liver carcinoma cell line), MCF7 (Human breast adenocarcinoma cell line), HeLa (Human epithelial carcinoma cell line), PC3 (Human prostate cancer cell line), NIH 3T3 (Mouse embryonic fibroblast cell line). This antibody also gave a positive signal in the following tissue lysates: Human Heart, Human Brain, Human Kidney, Human Liver.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).

靶标

  • 功能
    Strongly activates transcription when bound to HCFC1. Suppresses the expression of HSV proteins in cells infected with the virus in a HCFC1-dependent manner. Also suppresses the HCFC1-dependent transcriptional activation by CREB3 and reduces the amount of CREB3 in the cell. Able to down-regulate expression of some cellular genes in CREBZF-expressing cells.
  • 组织特异性
    In adults, expressed most abundantly in heart, liver and skeletal muscle, moderately abundant in kidney and pancreas, and barely detectable in lung. In fetal tissues, expressed most abundantly in kidney and very low amounts in heart, lung and liver.
  • 序列相似性
    Belongs to the bZIP family. ATF subfamily.
    Contains 1 bZIP domain.
  • 细胞定位
    Nucleus. Colocalizes at punctate nuclear structures with CREB3 and HCFC1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110034C16Rik antibody
    • 6330417B10Rik antibody
    • AI225749 antibody
    • AI606244 antibody
    • CREB/ATF bZIP transcription factor antibody
    • Crebzf antibody
    • FLJ94018 antibody
    • HCF-binding transcription factor Zhangfei antibody
    • Host cell factor-binding transcription factor Zhangfei antibody
    • LAZip antibody
    • LAZipII antibody
    • SHP-interacting leucine zipper protein antibody
    • SMILE antibody
    • ZF antibody
    • ZHANG_HUMAN antibody
    see all

Anti-Zhangfei antibody 图像

  • All lanes : Anti-Zhangfei antibody (ab28700) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human brain tissue lysate - total protein (ab29466)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : 293T Embryonic Kidney cell line (Human) Whole Cell Lysate
    Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 8 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 9 : PC3 whole cell lysate (ab3954)
    Lane 10 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    In the literature Zhangfei is also referred to as SMILE (SHP-interacting leucine zipper protein). The SMILE gene generates two isoforms, SMILE-L (long isoform of SMILE, 50–60 kDa) and SMILE-S (short isoform of SMILE, 40 kDa), and ab28700 predominantly recognizes the SMILE-L form.

实验方案

Anti-Zhangfei antibody (ab28700)参考文献

This product has been referenced in:
  • Chen F  et al. Construction and expression of lentiviral vectors encoding recombinant mouse CREBZF in NIH 3T3 cells. Plasmid 76C:24-31 (2014). Read more (PubMed: 25195838) »
  • López-Mateo I  et al. The transcription factor CREBZF is a novel positive regulator of p53. Cell Cycle 11:3887-95 (2012). Read more (PubMed: 22983008) »

See all 5 Publications for this product

Product Wall

Further to my previous message, our lab has retested this antibody and produced the attached results. Ab28700 primarily detects the Smile-L isoform. I hope this helps, please let me know if you need any additional information. All lanes : Anti-Zhangfei...

Read More

Thank you for contacting us. I apologize for the delay in my reply. Our lab provided the following explanation for the WB data: This seems to be a challenging target; in Biochem. J. (2008) 416 (463–473) they state: “SMILE (SHP-interact...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (breast)
Specification
breast
Fixative
formaline
Antigen retrieval step
Heat mediated
Permeabilization
No
Username

Dr. fathima sheriff

Verified customer

提交于 Jul 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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