The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
功能May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes.
组织特异性Widely expressed. Highly expressed in kidney, placenta and lung. Expressed at intermediate level in heart, brain, skeletal muscle, spleen and liver. Weakly expressed in colon, thymus and peripheral blood lymphocytes.
序列相似性Contains 1 FYVE-type zinc finger.
结构域The FYVE-type zinc finger is necessary and sufficient for its localization into early endosomes and mediates the association with PI3P.
细胞定位Cytoplasm. Early endosome membrane. Localized to early endosomes. Membrane-associated, probably via its association with phosphatidylinositol 3-phosphate.