The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 117 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Involved in postimplantation and gastrulation stages of development. Involved in the nucleocytoplasmic shuttling of STAU2. Binds to DNA and RNA.
Expressed in lung, liver, lymphocytes, heart, pancreas, placenta, brain and kidney.
Contains 1 DZF domain.
Nucleus. Cytoplasm. Cytoplasmic granule. Chromosome. Associated with chromosome foci in meiotic cells. Localizes in somatodendritic compartment of primary hippocampal neurons. Colocalizes with STAU2 in several cytosolic RNA granules.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling ZFR with ab90865 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining seen in the cytoplasm and nuclei of pinelocytes and intersticial cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - ZFR. Right - Merge.
Western blot - ZFR antibody (ab90865)
Anti-ZFR antibody (ab90865) at 1 µg/ml + HT1080 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution