Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 400-450 of Human ZFP64 (NP_060667.2).
Jurkat, HeLa, 293T and NIH 3T3 whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
Immunogen affinity purified
ab117787 was affinity purified using an epitope specific to ZFP64 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 74 kDa.
Use at 2-10 µg/mg of lysate.
ZFP64 belongs to the krueppel C
2H 2-type zinc-finger protein family. It contains 9 C 2H 2-type zinc fingers and may be involved in transcriptional regulation.
Western blot - Anti-ZFP64 antibody (ab117787)
All lanes : Anti-ZFP64 antibody (ab117787) at 0.04 µg/ml Lane 1 : 293T whole cell lysates at 50 µg Lane 2 : 293T whole cell lysates at 15 µg Lane 3 : Jurkat whole cell lysates at 50 µg Lane 4 : HeLa whole cell lysates at 50 µg Lane 5 : NIH 3T3 whole cell lysates at 50 µg Developed using the ECL technique Predicted band size : 74 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-ZFP64 antibody (ab117787)
Detection of ZFP64 by Western Blot of Immunprecipitate.
ab117787 at 1µg/ml staining ZFP64 in 293T whole cell lysate immunoprecipitated using ab117787 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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