• 产品名称Anti-Zfp57抗体
    参阅全部 Zfp57 一抗
  • 描述
    兔多克隆抗体to Zfp57
  • 特异性This antibody reacts with Zfp57
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A region within synthetic peptide: MFEQLKPIEP RDCWREARVK KKPVTFEDVA VNFTQEEWDC LDASQRVLYQ, corresponding to N terminal amino acids 1-50 of Human Zfp57

  • 阳性对照
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue IF/ICC: MEF1 cell line.



Our Abpromise guarantee covers the use of ab50944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 4 - 8 µg/ml.


  • 相关性Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

Anti-Zfp57 antibody 图像

  • ICC/IF image of ab50944 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50944, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Zfp57 antibody (ab50944) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50,000

    Predicted band size : 59 kDa
    Observed band size : 64,65,66 kDa (why is the actual band size different from the predicted?)
  • Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.Ab50944, at a concentration of 4µg/ml, staining Zpf57 in the epithelial cells of human kidney renal tubules. Magnification: x400.

Anti-Zfp57 antibody (ab50944)参考文献

ab50944 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Specification kidney
Blocking step Protein Block (Leica) as blocking agent for 5 minute(s) · Concentration: 0.4% · Temperature: 21°C
Fixative Formaldehyde

Ms. Eva Rodriguez Nunez

Verified customer

提交于 Aug 05 2015

Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab ...

Read More