概述

  • 产品名称Anti-Zfp57抗体
    参阅全部 Zfp57 一抗
  • 描述
    兔多克隆抗体to Zfp57
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Zfp57.

    (Peptide available as ab45340.)

  • 阳性对照
    • This antibody also gave a positive signal in the following Mouse Whole Cell Lysate: NIH 3T3 (ab7179) (data not shown)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab45341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with Mouse Zfp57 peptide (ab45340).

靶标

  • 相关性Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

Anti-Zfp57 antibody 图像

  • Anti-Zfp57 antibody (ab45341) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)


    Exposure time : 150 seconds

Anti-Zfp57 antibody (ab45341)参考文献

This product has been referenced in:
  • Riso V  et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res 44:8165-78 (2016). Read more (PubMed: 27257070) »
  • Baglivo I  et al. Genetic and epigenetic mutations affect the DNA binding capability of human ZFP57 in transient neonatal diabetes type 1. FEBS Lett 587:1474-81 (2013). ChIP ; Mouse . Read more (PubMed: 23499433) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. No, these antibodies have not been tested with overexpression or knockdown cells.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Our testing did show a band at this range in mouse brain lysate as well (http://www.abcam.com/Zfp57-antibody-ab45341.html#ab45341_1.jpg) that we were not able to identify. To our knowledge, this product has not...

Read More

Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab ...

Read More
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (Testes)
Total protein in input 20 µg
Specification Testes
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

提交于 Aug 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Uterus)
Specification Uterus
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"