概述

  • 产品名称
  • 描述
    兔多克隆抗体to Zfp57
  • 经测试应用
    适用于: ChIP, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse Zfp57.

    (Peptide available as ab45340.)

  • 阳性对照
    • This antibody also gave a positive signal in the following Mouse Whole Cell Lysate: NIH 3T3 (ab7179) (data not shown)

性能

应用

Our Abpromise guarantee covers the use of ab45341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 26728555
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Can be blocked with Mouse Zfp57 peptide (ab45340).

靶标

  • 相关性
    Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • bA145L22 antibody
    • bA145L22.2 antibody
    • C6orf40 antibody
    • TNDM1 antibody
    • Zfp 57 antibody
    • ZFP57 zinc finger protein antibody
    • Zinc Finger Protein 57 antibody
    • Zinc finger protein 57 homolog antibody
    • Zinc finger protein 698 antibody
    • ZNF698 antibody
    see all

图片

  • Anti-Zfp57 antibody (ab45341) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate (ab50253) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 57 kDa (why is the actual band size different from the predicted?)


    Exposure time : 150 seconds

实验方案

文献

This product has been referenced in:
  • Luo Z  et al. Regulation of the imprinted Dlk1-Dio3 locus by allele-specific enhancer activity. Genes Dev 30:92-101 (2016). CHIPseq ; Mouse . Read more (PubMed: 26728555) »
  • Riso V  et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res 44:8165-78 (2016). Read more (PubMed: 27257070) »

See all 6 Publications for this product

客户评价及客户问答

Thank you for contacting us. No, these antibodies have not been tested with overexpression or knockdown cells.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

Our testing did show a band at this range in mouse brain lysate as well (http://www.abcam.com/Zfp57-antibody-ab45341.html#ab45341_1.jpg) that we were not able to identify. To our knowledge, this product has not...

Read More

Thank you for contacting us. Unfortunately it is unlikely that ab45341 works in Human (poor sequence homology). Thank you for sending us the reference of the publication in which ab45341 was used. About ab50944, you mentioned that another lab ...

Read More
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (Testes)
Total protein in input
20 µg
Specification
Testes
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 Aug 02 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Uterus
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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