Anti-Zfp281抗体(ab89445)

概述

  • 产品名称Anti-Zfp281抗体
    参阅全部 Zfp281 一抗
  • 描述
    小鼠单克隆抗体to Zfp281
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Recombinant fragment: KLTSQKEQKN LESSTGFQIP SQELASQIDP QKDIEPRTTY QIENFAQAFG , corresponding to amino acids 787-894 of Human Zfp281

  • 阳性对照
    • A431 cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab89445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 97 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GC box binding zinc finger protein 1 antibody
    • GC-box-binding zinc finger protein 1 antibody
    • GZP1 antibody
    • Transcription factor ZBP-99 antibody
    • ZBP 99 antibody
    • ZBP99 antibody
    • zfp281 antibody
    • Zinc finger DNA binding protein 99 antibody
    • Zinc finger DNA-binding protein 99 antibody
    • Zinc finger protein 281 antibody
    • ZN281_HUMAN antibody
    • ZNF281 antibody
    • ZNP 99 antibody
    • ZNP 99 transcription factor antibody
    • ZNP99 antibody
    see all

Anti-Zfp281 antibody 图像

  • Anti-Zfp281 antibody (ab89445) at 1 µg/ml + A431 cell lysate at 50 µg

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • Anti-Zfp281 antibody (ab89445) at 1 µg/ml + recombinant immunogen at 0.2 µg

    Predicted band size : 97 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-Zfp281 antibody (ab89445)参考文献

ab89445 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (embryonic stem cells)
Loading amount 40 µg
Specification embryonic stem cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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提交于 Jul 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"