Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla
Synthetic peptide, corresponding to a region between amino acids 775-825 of Human Zfp281 (NP_036614.1).
HeLa and 293T whole cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: 7 to 8
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
Use at 2-10 µg/mg of lysate.
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
GC box binding zinc finger protein 1 antibody
GC-box-binding zinc finger protein 1 antibody
Western blot - Anti-Zfp281 antibody (ab112047)
All lanes : Anti-Zfp281 antibody (ab112047) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 97 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-Zfp281 antibody (ab112047)
Detection of Zfp281 by Western Blot of Immunprecipitate.
ab112047 at 1µg/ml staining Zfp281 in HeLa whole cell lysate immunoprecipitated using ab112047 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
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