Anti-Zfp219抗体- Aminoterminal end (ab71279)

概述

  • 产品名称Anti-Zfp219抗体- Aminoterminal end
    参阅全部 Zfp219 一抗
  • 描述
    兔多克隆抗体to Zfp219 - Aminoterminal end
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids 161-191 of Human Zfp219

  • 阳性对照
    • 293 cell line lysates.

性能

应用

Our Abpromise guarantee covers the use of ab71279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50 - 1/100. Detects a band of approximately 30, 55, 60, 77 kDa (predicted molecular weight: 77 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

Anti-Zfp219 antibody - Aminoterminal end 图像

  • Anti-Zfp219 antibody - Aminoterminal end (ab71279) at 1/60 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 77 kDa
    Observed band size : 77 kDa
    Additional bands at : 30 kDa,55 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • ab71279 at 1/50 dilution staining Zfp219 in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

Anti-Zfp219 antibody - Aminoterminal end (ab71279)参考文献

This product has been referenced in:
  • Takigawa Y  et al. The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9. J Cell Sci 123:3780-8 (2010). IHC-P ; Mouse . Read more (PubMed: 20940257) »

See 1 Publication for this product

Product Wall

I understand that you have a deadline coming up quickly and I really wish I could hurry the production process along. We do make every effort to produce the antibodies as quickly as possible, as you can imagine we can't sell the antibodies if they are ...

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Thanks for sending along the western blot and IHC data. That was very helpful. I agree it is odd that the same 2 intense bands are consistantly detected in all your blots. As well, the IHC staining should be largely nuclear and it is not. And, as you s...

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