Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Mouse Zfp219.
(Peptide available as ab31002.)
- This antibody gave a positive signal in the following whole cell lysates: F9; IOUD2.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/250. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).
May be involved in transcriptional regulation.
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers.
Information by UniProt
- Zinc finger protein 219 antibody
- ZN219_HUMAN antibody
- ZNF219 antibody
Western blot - Anti-Zfp219 antibody (ab31003)
All lanes :
Anti-Zfp219 antibody (ab31003) at 1/250 dilutionLane 1 :
F9 (Mouse embryonic carcinoma cell line) Whole Cell LysateLane 2 :
IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Lysate
Lysates/proteins at 20 µg/ml per lane.Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484
) at 1/3000 dilution
Developed using the ECL technique
Performed under reducing conditions.Predicted band size :
78 kDaObserved band size :
78 kDaAdditional bands at :
58 kDa,90 kDa. We are unsure as to the identity of these extra bands.Exposure time :
has not yet been referenced specifically in any publications.
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