概述

  • 产品名称Anti-ZFAND5抗体
  • 描述
    兔多克隆抗体to ZFAND5
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide to a region within: REDKITTPKT EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMC, corresponding to internal sequence amino acids 108-157 of Human ZFAND5 (NP_005998).

  • 阳性对照
    • HepG2 cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab86560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Could be involved in regulating NF-kappa-B activation and apoptosis. Is a potent inhibitory factor for osteoclast differentiation. Involved in protein degradation via the ubiquitin-proteasome system and plays a critical role in muscle atrophy. May act by anchoring ubiquitinylated proteins to the proteasome, playing a critical role in protein degradation.
  • 组织特异性Highly expressed in skeletal muscle. Expressed in fetal cochlea. Also expressed in infant brain, fetal heart, pancreatic islet, melanocyte, pineal gland, placenta, corneal stroma, and parathyroid tumor. Weakly expressed or undetectable in adult brain, heart, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood leukocytes. Expressed in rhabdomyosarcoma RD cells (at protein level).
  • 序列相似性Contains 1 A20-type zinc finger.
    Contains 1 AN1-type zinc finger.
  • 结构域The A20-type zinc finger directly binds polyubiquitin chains and associates with the 26S proteasome. The zinc-finger A20-type domain is essential for inhibition of NF-kappa-B activation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AN1-type zinc finger protein 5 antibody
    • ZA20D2 antibody
    • ZFAN5_HUMAN antibody
    • Zfand5 antibody
    • ZFAND5A antibody
    • zinc finger A20 domain containing 2 antibody
    • Zinc finger A20 domain containing protein 2 antibody
    • Zinc finger A20 domain-containing protein 2 antibody
    • zinc finger AN1 type domain 5 antibody
    • Zinc finger protein 216 antibody
    • ZNF216 antibody
    see all

Anti-ZFAND5 antibody 图像

  • Anti-ZFAND5 antibody (ab86560) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ICC/IF image of ab86560 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86560, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ZFAND5 antibody (ab86560)参考文献

This product has been referenced in:
  • He G  et al. The Protein Zfand5 Binds and Stabilizes mRNAs with AU-rich Elements in Their 3'-Untranslated Regions. J Biol Chem 287:24967-77 (2012). WB . Read more (PubMed: 22665488) »

See 1 Publication for this product

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