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Tissue/ cell preparation (Zebrafish): Lysate of zebrafish intestines.
The transparency of the juvenile zebrafish and its genetic advantages make it an attractive model for the study of intestinal differentiation and renewal. This antibody labels the brush border / glycocalyx at the apical surface of intestinal absorptive cells. This antibody is not reactive with goblet cells; thus 4E8 can be used as a marker for brush border cells.
Our Abpromise guarantee covers the use of ab73643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC - Wholemount||Use at an assay dependent concentration. PubMed: 23352790|
|ICC/IF||Use at an assay dependent concentration. PubMed: 24504339|