Anti-ZEB1抗体(ab81972)
Key features and details
- Goat polyclonal to ZEB1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-ZEB1抗体
参阅全部 ZEB1 一抗 -
描述
山羊多克隆抗体to ZEB1 -
宿主
Goat -
特异性
ab81972 is expected to recognize both reported isoforms (NP_001121600.1; NP_110378.3). -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse
预测可用于: Chicken, Hamster, Cow, Xenopus laevis, Cynomolgus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Human ZEB1 aa 309-323 (internal sequence). According to NP_001121600.1 (or 325-339 of NP_110378.3).
Sequence:C-RPQIRQKIENKPLQE
Database link: NP_001121600.1 -
阳性对照
- ICC/IF: U2OS cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81972于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 124 kDa.
A 1 hour primary incubation is recommended for this product. |
ICC/IF | (1) |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 0.05 - 0.2 µg/ml. Predicted molecular weight: 124 kDa. A 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
组织特异性
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
疾病相关
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
序列相似性
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 535183 Cow
- Entrez Gene: 21417 Mouse
- SwissProt: Q64318 Mouse
- Unigene: 3929 Mouse
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别名
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
图片
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Anti-ZEB1 antibody (ab81972) at 0.05 µg/ml + Mouse heart tissue lysate in ripa buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour -
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab81972 被引用在 7 文献中.
- Huang J et al. LncRNA SNHG15 regulates EGFR-TKI acquired resistance in lung adenocarcinoma through sponging miR-451 to upregulate MDR-1. Cell Death Dis 11:525 (2020). PubMed: 32655137
- Ni J et al. Human trophoblast-derived exosomes attenuate doxorubicin-induced cardiac injury by regulating miR-200b and downstream Zeb1. J Nanobiotechnology 18:171 (2020). PubMed: 33218341
- Liu H et al. MiR-194-5p inhibited metastasis and EMT of nephroblastoma cells through targeting Crk. Kaohsiung J Med Sci 36:265-273 (2020). PubMed: 31889432
- Chen J et al. The anti-inflammation, anti-oxidative and anti-fibrosis properties of swertiamarin in cigarette smoke exposure-induced prostate dysfunction in rats. Aging (Albany NY) 11:10409-10421 (2019). PubMed: 31739286
- Fu D et al. Hsa_circ_0057481 promotes laryngeal cancer proliferation and migration by modulating the miR-200c/ZEB1 axis. Int J Clin Exp Pathol 12:4066-4076 (2019). PubMed: 31933802
- Huang X et al. Adam12 and lnc015192 act as ceRNAs in breast cancer by regulating miR-34a. Oncogene N/A:N/A (2018). PubMed: 30042416
- Liu L et al. ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer. PLoS One 11:e0148774 (2016). WB . PubMed: 26882471