概述

  • 产品名称Anti-ZDHHC8抗体
    参阅全部 ZDHHC8 一抗
  • 描述
    山羊多克隆抗体to ZDHHC8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide:

    C-QRDHPQLKTPPSK

    , corresponding to internal sequence amino acids 708-720 of Human ZDHHC8

  • 阳性对照
    • Human Brain (Frontal Cortex) lysate.

性能

应用

Our Abpromise guarantee covers the use of ab59483 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明Peptide ELISA: antibody detection limit dilution 1:16,000.

    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 81 kDa). An additional band of 48kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Palmitoyltransferase involved in glutamatergic transmission. Mediates palmitoylation of ABCA1.
    • 序列相似性Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.
      Contains 1 DHHC-type zinc finger.
    • 结构域The DHHC domain is required for palmitoyltransferase activity.
    • 细胞定位Cytoplasmic vesicle membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DHHC 8 antibody
      • DHHC domain containing 8 antibody
      • DHHC-8 antibody
      • KIAA1292 antibody
      • Membrane associated DHHC8 zinc finger protein antibody
      • Probable palmitoyltransferase ZDHHC8 antibody
      • ZDHC8_HUMAN antibody
      • ZDHHC8 antibody
      • ZDHHCL1 antibody
      • Zinc finger DHHC domain containing protein 8 antibody
      • Zinc finger DHHC domain-containing protein 8 antibody
      • Zinc finger protein 378 antibody
      • ZNF378 antibody
      see all

    Anti-ZDHHC8 antibody 图像

    • All lanes : Anti-ZDHHC8 antibody (ab59483) at 0.1 µg/ml

      Lane 1 : Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer) without blocking with the immunising peptide
      Lane 2 : Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer) with blocking with the immunising peptide


      Predicted band size : 81 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.

    Anti-ZDHHC8 antibody (ab59483)参考文献

    ab59483 has not yet been referenced specifically in any publications.

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