Anti-ZDHHC15抗体(ab121203)

概述

  • 产品名称
    Anti-ZDHHC15抗体
  • 描述
    兔多克隆抗体to ZDHHC15
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: VSRNKTTLEA FCTPVFTSGP EKNGFNLGFI KNIQQVFGDK KKFWLIPIGS SPGDGHSFPM RSMNESQNPL LANEETWEDN EDDNQ, corresponding to internal sequence amino acids 238-322 of Human ZDHHC15.

  • 阳性对照
    • Human pancreas tissue; Human liver lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/250.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 功能
    Palmitoyltransferase specific for GAP43 and DLG4/PSD95.
  • 组织特异性
    Expressed in placenta, liver, lung, kidney, heart and brain.
  • 疾病相关
    Defects in ZDHHC15 are the cause of mental retardation X-linked type 91 (MRX91) [MIM:300577]. Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • 序列相似性
    Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • 结构域
    The DHHC domain is required for palmitoyltransferase activity.
  • 翻译后修饰
    Autopalmitoylated.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DHHC-15 antibody
    • Palmitoyltransferase ZDHHC15 antibody
    • UNQ1969/PRO4501 antibody
    • ZDH15_HUMAN antibody
    • Zdhhc15 antibody
    • Zinc finger DHHC domain-containing protein 15 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121203 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121203 at 1/5 dilution staining ZDHHC15 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.
  • All lanes : Anti-ZDHHC15 antibody (ab121203) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver cell lysate
    Lane 5 : Human tonsil cell lysate

文献

ab121203 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary hippocampal neurons)
Permeabilization
Yes - 0.3% triton X 100
Specification
primary hippocampal neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Bhavin Shah

Verified customer

提交于 Apr 09 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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