ZCH11 overexpression 293T裂解液(全细胞) (ab94144)

概述

  • 产品名称ZCH11 overexpression 293T裂解液(全细胞)
  • 描述
    ZCH11 overexpression裂解液
  • 常规说明ab94144 is a 293T cell transfected lysate in which Human ZCH11 has been transiently over-expressed using a pCMV-ZCH11 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. Similarity: Belongs to the DNA polymerase type-B-like family. Contains 3 CCHC-type zinc fingers. Contains 2 PAP-associated domains. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

相关产品

应用

Our Abpromise guarantee covers the use of ab94144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

ZCH11 overexpression 293T lysate (whole cell) 图像

  • ab94144 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-ZCH11 antibody (ab89165) at 1/500 dilution

    Lane 1 : ZCH11 overexpression 293T lysate (whole cell) (ab94144)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

ZCH11 overexpression 293T lysate (whole cell) (ab94144)参考文献

ab94144 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Questions for ab94144.
Please use the link above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"