概述

  • 产品名称Anti-ZCH11抗体
    参阅全部 ZCH11 一抗
  • 描述
    山羊多克隆抗体to ZCH11
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性

    预测可用于: Mouse, Rat, Cow, Dog, Human
  • 免疫原

    Synthetic peptide:

    RERCPHPPRGNVSE

    , corresponding to C terminal amino acids 1503-1516 of KIAA0191 (protein sequence according to BAA12105)

  • 阳性对照
    • Human lymph Node
  • 常规说明Principal Names - KIAA0191 protein GenBank Accession Number – BAA12105 LocusLink ID - 23318 Note:Please Click Here for Instructions For Use/Protocol


    A ubiquitously expressed gene with a predicted C2H2 zinc-finger at its N-terminal region.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody说明A ubiquitously expressed gene with a predicted C2H2 zinc-finger at its N-terminal region.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab9940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human ZCH11 peptide (ab23172). Approx 250-300 kDa and 30-35 kDa band seen in Raji Lysate. Please note that the band we consistently detect in western blots differs from the predicted size of 181kDa. Currently we cannot find an explanation for this in the literature. However, it has several consensus glycosylation sites. Glycosylations are known to affect migration of proteins on a SDS-PAGE.
IHC-P Use at an assay dependent dilution. In paraffin-embedded Human Lymph Node shows nuclear staining of select lymphocytes and staining of macrophages.

靶标

  • 功能Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation.
  • 序列相似性Belongs to the DNA polymerase type-B-like family.
    Contains 3 CCHC-type zinc fingers.
    Contains 2 PAP-associated domains.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp779C1943 antibody
    • EC 2.7.7.52 antibody
    • FLJ42878 antibody
    • KIAA0191 antibody
    • PAP associated domain containing 3 antibody
    • PAPD3 antibody
    • Terminal uridylyltransferase 4 antibody
    • TUT4 antibody
    • TUT4_HUMAN antibody
    • TUTase 4 antibody
    • ZCCHC 11 antibody
    • Zcchc11 antibody
    • Zinc finger CCHC domain containing 11 antibody
    • Zinc finger CCHC domain containing protein 11 antibody
    • Zinc finger CCHC domain-containing protein 11 antibody
    see all

Anti-ZCH11 antibody 图像

  • ab9940 staining ZCH11 in Human Lymph Node at 4 µg/ml. Steamed antigen retrieval with citrate buffer pH 6 was performed, antibody revealed with HRP-staining

Anti-ZCH11 antibody (ab9940)参考文献

ab9940 has not yet been referenced specifically in any publications.

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