Anti-ZCH11抗体(ab89165)
Key features and details
- Mouse polyclonal to ZCH11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZCH11抗体
参阅全部 ZCH11 一抗 -
描述
小鼠多克隆抗体to ZCH11 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human ZCH11. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human spleen tissue lysate; ZCH11 transfected 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89165于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa. |
靶标
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功能
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. -
序列相似性
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS. - Information by UniProt
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数据库链接
- Entrez Gene: 23318 Human
- Omim: 613692 Human
- SwissProt: Q5TAX3 Human
- Unigene: 655407 Human
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别名
- DKFZp779C1943 antibody
- EC 2.7.7.52 antibody
- FLJ42878 antibody
see all
图片
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Anti-ZCH11 antibody (ab89165) at 1 µg/ml + Human spleen tissue lysate at 50 µg
Predicted band size: 185 kDa
Observed band size: 185 kDa -
All lanes : Anti-ZCH11 antibody (ab89165) at 1 µg/ml
Lane 1 : ZCH11 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 185 kDa
数据表及文件
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Datasheet download
文献 (1)
ab89165 被引用在 1 文献中.
- Plé H et al. The repertoire and features of human platelet microRNAs. PLoS One 7:e50746 (2012). WB ; Human . PubMed: 23226537