Customer reviews and FAQs

概述

  • 产品名称Anti-ZCCHC9抗体
    参阅全部 ZCCHC9 一抗
  • 描述
    兔多克隆抗体to ZCCHC9
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: TTYNKRPLPA TSWEDMKKGS FEGTSQNLPK RKQLEANRLS LKNDAPQAKH KKNKKKKEYL NEDVNGFMEY LRQNSQMVHN GQI, corresponding to N terminal amino acids 9-91 of Human ZCCHC9.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human duodenum tissue; ZCCHC9 over-expression HEK293T lysate.

性能

应用

Our Abpromise guarantee covers the use of ab122470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 序列相似性Contains 4 CCHC-type zinc fingers.
  • Information by UniProt
  • 数据库链接
    • 别名
      • PPP1R41 antibody
      • Protein phosphatase 1 regulatory subunit 41 antibody
      • Zcchc9 antibody
      • ZCHC9_HUMAN antibody
      • Zinc finger CCHC domain containing protein 9 antibody
      • Zinc finger CCHC domain-containing protein 9 antibody
      • Zinc finger, CCHC domain containing 9 antibody
      see all

    Anti-ZCCHC9 antibody 图像

    • Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
    • Western blot - Anti-ZCCHC9 antibody (ab122470)
      Western blot - Anti-ZCCHC9 antibody (ab122470)
      All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilution

      Lane 1 : Negative control (vector only transfected HEK293T lysate)
      Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)

      Developed using the ECL technique

    Anti-ZCCHC9 antibody (ab122470)参考文献

    ab122470 has not yet been referenced specifically in any publications.

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