概述

  • 产品名称
    Anti-ZCCHC9抗体
  • 描述
    兔多克隆抗体to ZCCHC9
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: TTYNKRPLPA TSWEDMKKGS FEGTSQNLPK RKQLEANRLS LKNDAPQAKH KKNKKKKEYL NEDVNGFMEY LRQNSQMVHN GQI, corresponding to N terminal amino acids 9-91 of Human ZCCHC9.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human duodenum tissue; ZCCHC9 over-expression HEK293T lysate.

性能

应用

Our Abpromise guarantee covers the use of ab122470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

靶标

  • 序列相似性
    Contains 4 CCHC-type zinc fingers.
  • Information by UniProt
  • 数据库链接
    • 别名
      • PPP1R41 antibody
      • Protein phosphatase 1 regulatory subunit 41 antibody
      • Zcchc9 antibody
      • ZCHC9_HUMAN antibody
      • Zinc finger CCHC domain containing protein 9 antibody
      • Zinc finger CCHC domain-containing protein 9 antibody
      • Zinc finger, CCHC domain containing 9 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC9 antibody (ab122470)
      Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus and nucleoli. Recommended concentration of ab122470 1-4 µg/ml. Cells treated with PFA/Triton X-100.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC9 antibody (ab122470)
      ab122470, at 1/500 dilution, staining ZCCHC9 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
    • Western blot - Anti-ZCCHC9 antibody (ab122470)
      Western blot - Anti-ZCCHC9 antibody (ab122470)
      All lanes : Anti-ZCCHC9 antibody (ab122470) at 1/250 dilution

      Lane 1 : Negative control (vector only transfected HEK293T lysate)
      Lane 2 : Over-expression lysate (co-expressed with a C-terminal myc-DDK tag [~3.1 kDa] in HEK293T cells)

      Developed using the ECL technique.

    文献

    ab122470 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab122470？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    There are currently no Customer reviews or Questions for ab122470.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册