Anti-ZCCHC6抗体(ab76901)

概述

  • 产品名称Anti-ZCCHC6抗体
  • 描述
    小鼠多克隆抗体to ZCCHC6
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human ZCCHC6 protein (aa 1-412 of AAH32456 or Swissprot id Q5VYS8-5).

  • 阳性对照
    • Human liver tissue lysate; ZCCHC6 transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab76901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: 1/50.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight, isoform 1: 171 kDa, isoform 5: 48 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性ZCCHC6 contains 3 CCHC-type zinc fingers.
    • 数据库链接
    • 别名
      • KIAA1711 antibody
      • MGC38585 antibody
      • PAP associated domain containing 6 antibody
      • PAPD6 antibody
      • Zinc finger CCHC domain containing 6 antibody
      see all

    Anti-ZCCHC6 antibody 图像

    • Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml + human liver tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 171 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-ZCCHC6 antibody (ab76901) at 5 µg/ml

      Lane 1 : ZCCHC6 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 171 kDa
      Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    • ab76901 staining ZCCHC6 in human breast cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent antigen retrieval in 0.1 sodium citrate buffer. The primary antibody was used at 1/50 dilution and incubated with sample for 2 hours. The DAB staining method was used for detection. 

    Anti-ZCCHC6 antibody (ab76901)参考文献

    ab76901 has not yet been referenced specifically in any publications.

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