Anti-ZCCHC12抗体(ab74774)

概述

  • 产品名称Anti-ZCCHC12抗体
    参阅全部 ZCCHC12 一抗
  • 描述
    兔多克隆抗体to ZCCHC12
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    A region within synthetic peptide: AREVMRVLQA TNPNLSVADF LRAMKLVFGE SESSVTAHGK FFNTLQAQGE, corresponding to amino acids 94-143 of Human ZCCHC12

  • 阳性对照
    • HepG2 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab74774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Transcriptional coactivator in the bone morphogenetic protein (BMP)-signaling pathway. It positively modulates BMP signaling by interacting with SMAD1 and associating with CBP in the transcription complex. It contributes to the BMP-induced enhancement of cholinergic-neuron-specific gene expression.
  • 序列相似性Belongs to the ZCCHC12 family.
    Contains 1 CCHC-type zinc finger.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2810028A01Rik antibody
    • FLJ16123 antibody
    • Paraneoplastic Ma antigen family member 7A antibody
    • PNMA7A antibody
    • SIZN antibody
    • SIZN1 antibody
    • Smad interacting zinc finger protein 1 antibody
    • Smad-interacting zinc finger protein 1 antibody
    • Zcchc12 antibody
    • ZCH12_HUMAN antibody
    • Zinc finger CCHC domain containing 12 antibody
    • Zinc finger CCHC domain containing protein 12 antibody
    • Zinc finger CCHC domain-containing protein 12 antibody
    see all

Anti-ZCCHC12 antibody 图像

  • Anti-ZCCHC12 antibody (ab74774) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.

Anti-ZCCHC12 antibody (ab74774)参考文献

ab74774 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (bone marrow derived mesenchymal stem cell)
Loading amount 15 µg
Specification bone marrow derived mesenchymal stem cell
Treatment 20 uM specific compound for 48hrs
Gel Running Conditions Reduced Denaturing (gel 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"