Synthetic peptide, corresponding to a region between residue 50 and 100 of human ZC3H8 using the numbering given in entry NP_115883.2 (GeneID
Whole cell lysate from HeLa or 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 99% Tris citrate/phosphate, 0.09% Sodium chloride
Note: pH: 7-8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 34 kDa.
Use at 2-5 µg/mg of lysate.
Western blot - Anti-ZC3H8 antibody (ab113260)
All lanes : Anti-ZC3H8 antibody (ab113260) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 34 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-ZC3H8 antibody (ab113260)
Lane 1: 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab113260 at 6µg/mg lysate and 20% IP was loaded on gel.
Lane 2: same, with a control IgG.
For WB ab113260 was used at 1µg/ml. Detection:
chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"