概述

  • 产品名称Anti-ZC3H7B抗体
    参阅全部 ZC3H7B 一抗
  • 描述
    兔多克隆抗体to ZC3H7B
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: LPHDESVTQL GQELAQKLGL RVRKAYKRPQ ELETFSLLSN GTAAGVADQG , corresponding to amino acids 146-195 of Human ZC3H7B

  • 阳性对照
    • Liver lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 4 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 112 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-ZC3H7B antibody 图像

  • Anti-ZC3H7B antibody (ab66758) at 4 µg/ml + Liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 112 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa,40 kDa. We are unsure as to the identity of these extra bands.

Anti-ZC3H7B antibody (ab66758)参考文献

ab66758 has not yet been referenced specifically in any publications.

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