概述

  • 产品名称Anti-ZC3H3抗体
    参阅全部 ZC3H3 一抗
  • 描述
    兔多克隆抗体to ZC3H3
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 898-948 of Human ZC3H3, (NP_055932.2)

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.
  • 常规说明Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 序列相似性Contains 5 C3H1-type zinc fingers.
    • Information by UniProt
    • 数据库链接
    • 别名
      • KIAA0150 antibody
      • ZC3H3 antibody
      • ZC3H3_HUMAN antibody
      • ZC3HDC3 antibody
      • Zinc finger CCCH domain containing protein 3 antibody
      • Zinc finger CCCH domain-containing protein 3 antibody
      • Zinc finger CCCH type containing 3 antibody
      • Zinc finger CCCH type domain containing 3 antibody
      see all

    Anti-ZC3H3 antibody 图像

    • All lanes : Anti-ZC3H3 antibody (ab86416) at 0.04 µg/ml

      Lane 1 : HeLa cell lysate at 50 µg
      Lane 2 : HeLa cell lysate at 15 µg
      Lane 3 : HeLa cell lysate at 5 µg
      Lane 4 : 293T cell lysate at 50 µg


      Predicted band size : 101 kDa
      Observed band size : 117 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 71 kDa. We are unsure as to the identity of these extra bands.
    • Detection of Human ZC3H3 by Immunoprecipitation on Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86416 at 3 ug/mg lysate. Subsequent WB analysis was performed using ab86416 at 1ug/ml.

    Anti-ZC3H3 antibody (ab86416)参考文献

    ab86416 has not yet been referenced specifically in any publications.

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