概述

  • 产品名称Anti-ZC3H13抗体
    参阅全部 ZC3H13 一抗
  • 描述
    兔多克隆抗体to ZC3H13
  • 经测试应用适用于: ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1175 and 1225 of Human ZC3H13 (NP_055885.2)

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and NIH3T3 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab70802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/400.
IP Use at 2 µg/mg of lysate.
WB 1/2000 - 1/10000. Detects a band of approximately 240 kDa (predicted molecular weight: 197 kDa).

靶标

  • 序列相似性Contains 1 C3H1-type zinc finger.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2600010B19Rik antibody
    • 3110050K21Rik antibody
    • 4930570G11Rik antibody
    • C87618 antibody
    • DKFZp434D1812 antibody
    • FLJ35669 antibody
    • KIAA0853 antibody
    • ZC3H13 antibody
    • ZC3HD_HUMAN antibody
    • Zinc finger CCCH domain-containing protein 13 antibody
    • zinc finger CCCH type containing 13 antibody
    see all

Anti-ZC3H13 antibody 图像

  • All lanes : Anti-ZC3H13 antibody (ab70802) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 197 kDa
    Observed band size : 240 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,85 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of ZC3H13 by Western Blot of Immunoprecipitate.
    ab70802 at 1µg/ml staining ZC3H13 in HeLa whole cell lysate previously immunoprecipitated using ab70802 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: chemiluminescence with exposure time of 30 seconds.
  • ab70802 (1/400) staining ZC3H13 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% TritonX100/PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.

    See Abreview

Anti-ZC3H13 antibody (ab70802)参考文献

This product has been referenced in:
  • Horiuchi K  et al. Identification of Wilms' tumor 1-associating protein complex and its role in alternative splicing and the cell cycle. J Biol Chem 288:33292-302 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24100041) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

The concentration of ab70802 is 1 mg/ml. Yes, the recommended antibody dilution is 1/2000 - 1/10000. But the antibody can be used at other dilutions as well, dependenton the samples being studied.

As secon...

Read More

Thank you for contacting us.

The following WB protocol has been used with this antibody, togetherwitha 3-8% SDS Precast gel and HeLa whole cell lysate.

Western Blot Protocol

Reagents Needed:

20X Running Buffer <...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Nov 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"