Anti-ZC3H12D抗体(ab100862)
Key features and details
- Rabbit polyclonal to ZC3H12D
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-ZC3H12D抗体 -
描述
兔多克隆抗体to ZC3H12D -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
A 16 amino acid peptide from the intermediate residues of Human ZC3H12D protein (NP_997243).
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阳性对照
- WB: Fetal small intestine lysate and fetal spleen lystae IHC-P: Fetal small intestine
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:Add 200 ul of sterile distilled water -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100862于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 58 kDa.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 58 kDa. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
May regulate cell growth likely by suppressing RB1 phosphorylation (PubMed:19531561). May function as RNase and regulate the levels of target RNA species (Potential). In association with ZC3H12A enhances the degradation of interleukin IL-6 mRNA level in activated macrophages (PubMed:26134560). Serve as a tumor suppressor in certain leukemia cells (PubMed:17210687). Overexpression inhibits the G1 to S phase progression through suppression of RB1 phosphorylation (PubMed:19531561). -
组织特异性
Expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines. -
疾病相关
A chromosomal aberration involving ZC3H12D may be the cause of the transformation of follicular lymphoma (FL) to diffuse large B-cell lymphoma (DLBCL). Translocation t(2;6)(p12;q25) with IGK. Resulting protein may not be expressed. -
序列相似性
Belongs to the ZC3H12 family.
Contains 1 C3H1-type zinc finger. -
细胞定位
Cytoplasm. Nucleus; Cytoplasm. Localized as discrete granules. Colocalized with mRNA-processing body markers, AGO2 and DCP1A, but not with a stress granule maker, TIA1, in the cytoplasm and Cytoplasm, P-body. Colocalizes with ZC3H12A in GW bodies (GWBs) (PubMed:26134560). - Information by UniProt
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数据库链接
- Entrez Gene: 340152 Human
- Omim: 611106 Human
- SwissProt: A2A288 Human
- Unigene: 632618 Human
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别名
- C6orf95 antibody
- ZC3H12D antibody
- FLJ00361 antibody
see all
图片
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All lanes : Anti-ZC3H12D antibody (ab100862) at 1/500 dilution
Lane 1 : Human fetal small intestine lysate
Lane 2 : Human fetal spleen lysate
Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted? -
ab100862, at a 1/100 dilution, staining ZC3H12D in formalin-fixed, paraffin-embedded Human fetal small intestine tissue, showing cytoplasmic staining, by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100862 被引用在 2 文献中.
- Ye J et al. Long non-coding RNA FOXP4-AS1 facilitates the biological functions of hepatocellular carcinoma cells via downregulating ZC3H12D by mediating H3K27me3 through recruitment of EZH2. Cell Biol Toxicol 38:1047-1062 (2022). PubMed: 34545456
- Huang S et al. The putative tumor suppressor Zc3h12d modulates toll-like receptor signaling in macrophages. Cell Signal : (2011). IHC-P ; Mouse . PubMed: 22036805